lectin conjugated with biotin (Vector Laboratories)
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Vector Laboratories
lectin conjugated with biotin
Lectin Conjugated With Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+conjugated+concanavalin+a/Biotinylated+Concanavalin+A+(Con+A)/bio_rxiv__2025__08__12__669888-218-17-21
Average 94 stars, based on 302 article reviews
Lectin Conjugated With Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+conjugated+concanavalin+a/Biotinylated+Concanavalin+A+(Con+A)/bio_rxiv__2025__08__12__669888-218-17-21
Average 94 stars, based on 302 article reviews
lectin conjugated with biotin - by Bioz Stars,
2026-09
94/100 stars
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Incubation:Article Title: Synthetic arrest of Man 5 GlcNAc 2 -PP-Dol increases procyclin mRNA level and induces cell death in the bloodstream form Trypanosoma brucei brucei. Article Snippet: The biosynthesis of N-linked glycan precursors in the endoplasmic reticulum is important for many eukaryotes.. In particular, the synthesis of Man5GlcNAc2-PP-dolichol (M5-DLO) at the cytoplasmic face of the endoplasmic reticulum is essential for maintaining cellular functions.. In Trypanosoma brucei, the unicellular organism that causes African trypanosomiasis, homologs of the mannosyltransferases ALG2 and ALG11, which are involved in the biosynthesis of M5-DLO, are found, but the effects of their deletion on cells remain unknown. Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures Article Snippet: .. Blots were then stripped using stripping buffer at 4 °C overnight, and then washed and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Tools and tactics to define specificity of metabolic chemical reporters. Article Snippet: .. Then, the blots were stripped using stripping buffer at 4 °C overnight, washed, and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Establishment of fetomaternal tolerance through glycan-mediated B cell suppression Article Snippet: Immunoblotting for Neu5Gc was performed after overnight blocking with Neu5Gc assay blocking solution (BioLegend). .. For lectin immunoblots, the membranes were blocked with 1% BSA in 0.25 M Tris-Cl, 0.5 M NaCl, 0.5% NP-40, pH 8.0, then incubated overnight at 4 °C in this same buffer without BSA but instead containing 0.5 μg ml −1 biotin-conjugated S. nigra lectin (SNA-I; Elderberry Bark, EY Laboratories), 1 μg ml −1 biotin-conjugated M. amurensis lectin I (MAL-I, Vector Laboratories) or 0.5 μg ml −1 Article Title: Tools and tactics to define specificity of metabolic chemical reporters Article Snippet: .. Then, the blots were stripped using stripping buffer at 4 °C overnight, washed, and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Selective bioorthogonal probe for N-glycan hybrid structures. Article Snippet: .. Blots were then stripped using nitro stripping buffer for nitrocellulose membranes (Li-Cor, cat. no. 928-40030) at room temperature for 15 min, and then washed and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Stripping Membranes:Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures Article Snippet: .. Blots were then stripped using stripping buffer at 4 °C overnight, and then washed and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Tools and tactics to define specificity of metabolic chemical reporters. Article Snippet: .. Then, the blots were stripped using stripping buffer at 4 °C overnight, washed, and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Tools and tactics to define specificity of metabolic chemical reporters Article Snippet: .. Then, the blots were stripped using stripping buffer at 4 °C overnight, washed, and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Selective bioorthogonal probe for N-glycan hybrid structures. Article Snippet: .. Blots were then stripped using nitro stripping buffer for nitrocellulose membranes (Li-Cor, cat. no. 928-40030) at room temperature for 15 min, and then washed and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Blocking Assay:Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures Article Snippet: .. Blots were then stripped using stripping buffer at 4 °C overnight, and then washed and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Tools and tactics to define specificity of metabolic chemical reporters. Article Snippet: .. Then, the blots were stripped using stripping buffer at 4 °C overnight, washed, and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Tools and tactics to define specificity of metabolic chemical reporters Article Snippet: .. Then, the blots were stripped using stripping buffer at 4 °C overnight, washed, and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Article Title: Selective bioorthogonal probe for N-glycan hybrid structures. Article Snippet: .. Blots were then stripped using nitro stripping buffer for nitrocellulose membranes (Li-Cor, cat. no. 928-40030) at room temperature for 15 min, and then washed and blocked with Odyssey PBS blocking buffer at room temperature for 1 h. The blots were then incubated with Western Blot:Article Title: Establishment of fetomaternal tolerance through glycan-mediated B cell suppression Article Snippet: Immunoblotting for Neu5Gc was performed after overnight blocking with Neu5Gc assay blocking solution (BioLegend). .. For lectin immunoblots, the membranes were blocked with 1% BSA in 0.25 M Tris-Cl, 0.5 M NaCl, 0.5% NP-40, pH 8.0, then incubated overnight at 4 °C in this same buffer without BSA but instead containing 0.5 μg ml −1 biotin-conjugated S. nigra lectin (SNA-I; Elderberry Bark, EY Laboratories), 1 μg ml −1 biotin-conjugated M. amurensis lectin I (MAL-I, Vector Laboratories) or 0.5 μg ml −1 |